Osteogenic differentiation of human mesenchymal stem cells (hMSCs) within a perfusion bioreactor system. Homo sapiens

hMSCs were cultured for eight days within perfused three-dimensional matrices under conditions of oscillatory, continuous or static fluid flow of osteogenic media. Overall design: Total RNA was obtained from hMSC samples seeded within porous glass scaffolds following eight days exposure to either a high flow rate oscillatory (10L/24hrs), low flow rate oscillatory (1L/24hrs), continuous (1mL/24hrs) or a static (0mL/24hrs) flow rate condition. 3 RNA samples from each flow rate condition were analysed.