Near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of mouse epididymis (jrc_mus-epididymis -1)

<b>Sample: </b>Wild-type, 6 months old mouse, strain: C57BL/6 from Jackson Lab<b>Sample Description: </b>A dataset featuring isotropic 3D electron microscopy data of the mouse epididymis (jrc_mus-epididymis-2). The sample was obtained from a 6-month-old, wild-type C57BL/6 mouse strain sourced from Jackson Laboratory. Mouse euthanasia was performed as IACUC guidelines followed by immersion fixation in glutaraldehyde and vibratome section. After progressive lowering of temperature dehydration and <i>en bloc</i> staining with heavy metal, the sample was subject to infiltration with acetone and Durcupan resin, and polymerized in 60°C oven. The sample was provided by Zhiyuan Lu (HHMI/Janelia), prepared for imaging by Zhiyuan Lu, and imaged by Wei Qiu (HHMI/Janelia). Post-processing was conducted by Eric Trautman and Stephan Preibisch (HHMI/Janelia). This dataset offers a comprehensive view of the mouse epididymis' cellular architecture and facilitates further research in reproductive biology.<b>Protocol: </b>Mouse euthanasia was performed as IACUC guidelines followed by immersion fixation in glutaraldehyde and vibratome section. After progressive lowering of temperature dehydration and <i>en bloc</i> staining with heavy metal, the sample was subject to infiltration with acetone and Durcupan resin, and polymerized in 60°C oven.<b>Contributions</b>: Sample provided by Zhiyuan Lu (HHMI/Janelia), prepared for imaging by Zhiyuan Lu (HHMI/Janelia), and imaged by Wei Qiu (HHMI/Janelia) and post-processing by Eric Trautman and Stephan Preibisch (HHMI/Janelia).<b>Acquisition ID:</b> jrc_mus-epididymis-1<b>Voxel size (nm):</b> 4.0 x 4.0 x 4.0 (X, Y, Z)<b>Data dimensions (µm): </b>20.8 x 20.2 x 7.1 (X, Y, Z)<b>Imaging start date:</b> 2022-07-13<b>Dataset URL: </b>s3://janelia-cosem-datasets/jrc_mus-epididymis-1/jrc_mus-epididymis-1.zarr/recon-1/em/<b>Visualization Website</b>: https://openorganelle.janelia.org/datasets/jrc_mus-epididymis-1<b>Imaging duration (days): </b>4<b>Landing energy (eV):</b> 1<b>Imaging current (nA):</b> .25<b>Scanning speed (MHz):</b> .200