c-Myc supports polyploidy and prevents senescence in the murine placenta [RNA-seq]

To understand differential gene expression between wildtype and c-Myc null mouse trophoblast giant cells. Overall design: TGCs from 9.5 dpc stage were dissected as mentioned previously (Hannibal and Baker, 2016) cold PBS and embryos were used for genotyping. Total RNA from TGCs was isolated using TRIzol (#15596026, Thermo Fisher Scientific). rRNA was depleted using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB # E6310L). cDNA synthesis was performed using NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7760S) and NEBNext Multiplex Oligos for Illumina (Index Primer Set 1) (NEB # E7335S). Sequencing was performed using an Illumina NextSeq 500 with 75bp single read runs.