Gene expression profiling in two dimensional and extracellular matrix based three-dimensional cultures of lung and breast cancer cells

A549 and MDA-MB-231 cells were cultured in 2D and Matrigel based 3D culture for 4 days. Total RNA was extracted using Trizol. RNA-SEQ was carried out to profile the gene expression in both culture conditions. The goal of this experiment was to profie the genes that are regulated by extracellular matrix in cancer cells. Gene expression was probed globally using RNA-SEQ.