Bulk transcriptomic profiling of pluripotent stem cell-derived alveolar type 2 cells (iAT2s) cultured in spheres and at air-liquid interface and human HTII-280-sorted AT2s from a pediatric and adult donor lungs cultured in spheres

We performed bulk transcriptomic profiling of pluripotent stem cell-derived alveolar type 2 cells (iAT2s) and HTII-280-sorted human AT2s cultured with CK+DCI media. We used previously described distal lung directed differentiation protocol to generate alveolar type 2 cells (iAT2s). We used the SPC2-ST-B2 (“SPC2”) line containing a SFTPC-tdTomato knock in reporter. iAT2s were cultured in 3D spheres and at air-liquid interface. Human AT2s were HTII-280-sorted from a pediatric donor lung (13 months old) and an adult, nonsmoker human lung (32 years old) and cultured as spheres in the presence of MRC5s. After deconvolution, we find that iAT2s cultured in ALI correlate more closely to primary cultured AT2s than do iAT2s cultured in 3D spheres on the basis of lung epithelial gene expression. Overall design: Bulk transcriptomic profiling of pluripotent stem cell-derived alveolar type 2 cells (iAT2s) cultured in spheres and at air-liquid interface and human HTII-280-sorted AT2s from a pediatric and adult donor lungs cultured in spheres