Transcriptome profile changes during differentiation of avian satellite cells.

This study focused on a complex transcriptomic comparison of undifferentiated, differentiated and suspension cultured myosatellite cells, aiming to determine the effects of different culture methods on their transcriptome. Modern next-generation sequencing (RNAseq) was used to determine the levels of transcripts in cultures cell samples. Then differential expression and pathway analyses were performed using bionformatical methods. Differential regulation of gene expression, as well as significant enrichment and modulation of pathway activity, suggested that suspension culture potentially promotes the ex vivo associated loss of physiological characteristics and gain of plasticity. Overall design: Examination of 3 samples: undifferentiated, differentiated and suspended satellite cells.