Dach1 downregulation marks a lymphoid-primed progenitor in early haematopoiesis (2X96 cells)
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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A classical view of blood cell development is that multipotent haematopoietic stem and progenitor cells (HSPCs) become lineage-restricted at defined stages. The Linâc-kit+Sca1+Flt3+ stage, termed lymphoid-primed multipotent progenitors (LMPPs), have lost megakaryocyte and erythroid potential but are heterogeneous in their fate. Through single cell RNA-sequencing, we identify heterogeneous expression of Dach1 and associated genes in this fraction where it co-expressed with myeloid/stem genes but inversely correlated with lymphoid genes. Through generation of Dach1-GFP reporter mice, we identify a transcriptionally and functionally unique Dach1â subpopulation within LMPPs with lymphoid potential but devoid of myeloid potential. We term these âlymphoid-primed progenitorsâ, or LPPs. These findings define the earliest branch point of lymphoid development in haematopoiesis and a means for their prospective isolation.
提供机构：
Walter and Eliza Hall Institute of Medical Research
创建时间：
2022-02-20
