Altered nanoparticle uptake by lung carcinoma cells when stimulated with epidermal growth factor

This dataset provides the raw data supporting the paper "Altered nanoparticle uptake by lung carcinoma cells when stimulated with epidermal growth factor". The focus of the study was to investigate the uptake of two different sizes of silica NPs and gold NPs in lung epithelial cells A549 in the presence of epidermal growth factor (EGF). 

The data set includes:

Screening for EGF receptor using western blot and confocal microscopy (Figure 1 and Figure S1)

Investigating expression of RAC1/CDC42 proteins upon EGF stimulation using Western blot (Figure 2)

Investigating expression of RAC1 gene upon EGF stimulation using RT-qPCR (Figure S2)

Evaluating uptake of endocytic markers upon EGF stimulation using confocal laser scanning microscopy (Figure 3, Figure S4) and flow cytometry (Figure 3)

Nanoparticle characterization using TEM (Figure 4, Figure S6) and UV-Vis (Figure S5, Figure S6)

Evaluating silica nanoparticle uptake upon EGF stimulation using confocal laser scanning microscopy (Figure 5, Figure S8) and flow cytometry (Figure 5)

Evaluating gold nanoparticle uptake upon EGF stimulation using dark-field microscopy and ICP-AES (Figure 6)

Investigating expression of c-MYC gene upon EGF stimulation using RT-qPCR (Figure 6)

Cell viability results, analysed via lactate dehydrogenase assay (Figure S3) and MTS assay (Figure S9)

Raw integrated density data from dark-field images (Figure S9)