XIST modulates mammary stem cell homeostasis and tumor fate [WT and KO RNA-seq]

Disruption of XIST expression in mammary epithelial cells leads to chromatin changes on the inactive X chromosome, transcriptional reactivation of X-linked genes, and defective luminal differentiation. Overall design: Cells from both luminal (ML) and mammary stem cell (MaSC) compartments were isolated from wild-type (WT) and XIST-KO HMLE (Human Mammary Epithelial cells) by FACS sorting based on their positivity for the cell surface antigen ESA (Epithelial Specific Antigen) (ML: ESA+; MaSC: ESA-). Three replicates were generated per compartment and genotype. Total RNA was isolated and sequenced to perform gene expression and allele-specific analyses.