Synura

Illustrative figure. CyFlow FCM outputs of two chrysophyte algae of the genus Synura - S. americana with higher DNA content (3.69 pg) and S. leptorhabda with lower DNA content (0.21 pg) and its plant standards. Note clearly visible peaks with only minor background noise on both fluorescence histogram (a) and fluorescence vs. side scatter plot (b) in case of the first sample analysis. Conversely, higher amount of debris is present in the second analysed sample (c) with the sample DNA content approaching the limits of resolution for CyFlow instrument, yet with peaks still sufficiently separated on fluorescence vs. side scatter plot (d); unpublished data.
Conclusion:
The background noise is more pronounced in sample possessing lower DNA content. The CV values are 1.89 % (sample Synura americana) and 1.93 % (reference standard Solanum pseudocapsicum) and 5.14 % (sample Synura leptorhabda) and 1.82 % (reference standard Carex acutiformis).