Hordeum vulgare subsp. vulgare cultivar 'Morex' assembly (Hybrid assembly with Wengan)

Sequence assembly of large and repeat-rich plant genomes has been challenging, requiring substantial compute resources and often several complementary sequencing and mapping approaches. The recent development of fast and accurate long-read sequence assembly by circular consensus sequencing (CCS) on the PacBio platform may greatly increase the scope of plant pan-genome projects. Here, we compare current long-read sequencing platforms regarding their ability to rapidly generate contiguous sequence assemblies in pan-genome studies. PacBio CLR reads were used to scaffold the TRITEX contig assembly reported by Monat et al. (2019) with Wengan v0.1. Sequences were provided by the HudsonAlpha Genome Sequencing Center.