L. reuteri (LR) qPCR specificity test (CT values and melting curve analysis) using non-target strains from various sources, purchased from culture collections and isolated from intestinal samples.

CT cycle threshold.Tm melting temperature.MC melting curve.aDSMZ - Deutsche Sammlung von Mikroorganismen und Zellkultur.btarget strain and SSH tester strain.cLMG – Belgian coordinated collections of microorganisms.Ttype strain and SSH driver strain.n.a. no amplification.b.t. below threshold (33%).“-“ MC was either below threshold, showed the formation of one or more products, or showed a shift in Tm>1°C compared to Tm of LR (78.7°C).“+” MC properties were identical to LR.*melting curves not reproducible.