GROQ-seq Function Measurements for LacI, RamR, and VanR Transcription Factor Libraries — LMSF

This dataset contains GROQ-seq functional measurements of LacI, RamR, and VanR transcription factor variant libraries (SSVL, epPCR, and SS) assayed against their cognate wild-type operator sequences. This run of the assay was performed at the Living Measurement Systems Foundry (LMSF) at the National Institute of Standards and Technology. A companion dataset was collected at the Design, Automation, Manufacturing, and Processes (DAMP) Laboratory at Boston University, demonstrating cross-site reproducibility of the platform. Part of a broader effort to generate sequence → function data for allosteric transcription factors with applications in synthetic biology, biosensors, and therapeutics.