Polarization Measurements Of Doc-Dependent Ipab-Ipad Interactions
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The binding affinity between each of the engineered IpaD alanine mutants and the stable IpaB<sup>28-226</sup> construct was measured using fluorescence polarization. Here, the DOC effect on binding affinity between IpaB and the engineered IpaD π-helix mutants was quantified by holding IpaB<sup>28-226</sup>-Alexa568 concentration constant while the concentration of IpaD or engineered IpaD mutant was titrated from 0-10 μM with identical conditions then tested in the presence of 1 mM DOC.
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Zenodo创建时间：
2017-11-06
