Rhodococcus erythropolis and Pseudomonas aeruginosa coculture in control groups

Raw data from three parallel transcriptomes of <i>Rhodococcus erythropolis </i>and <i>Pseudomonas aeruginos</i>a coculture in 0 mM Al³⁺. The quality of the extracted RNA was assessed using a Nanodrop 2000 and an Agilent 4200 Tape Station bioanalyzer. Only RNA samples with an RNA integrity value (RIN) ≥7 were selected for cDNA library construction. The cDNA library fragments were purified using the AMPure XP system to ensure a preferred length of 400-500 bp. The number of PCR cycles was adjusted to 15, and the final amplified library was quality checked using a Bioanalyzer 2100 system. Finally, an equimolar library was constructed using the Kapa-sybr FAST qPCR Kit Light Cycler 480 and a reference standard from Kapa Biosystems.Each library was sequenced in paired-end mode using the TruSeq SBS kit v3-HS with a read length of 2 × 76 bp on the HiSeq2000 instrument (Illumina) according to the manufacturer's protocol for mRNA sequencing experiments. The <i>R. erythropolis</i> and<i> P. aeruginosa </i>genomes (GenBank assembly accession numbers: GCA_001715845.1 and GCA_016743035.1) were used as the reference genome.