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Systematic assessment of burst impurity in confocal-based single-molecule fluorescence detection using Brownian motion simulations - photon timetag simulation files

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NIAID Data Ecosystem2026-03-11 收录
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https://zenodo.org/record/3228993
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Attached are the photon timestamp and channels simulated for different 3D diffusing molecules simulations at different conditions (simulation was performed by PyBroMo). Each of the files has, in its name, a code. The meaning of the codes are as following: f32445 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a numerical PSF model  a01f8f - 15 molecules at a concentration of 31 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a numerical PSF model 9ff667 - 15 molecules at a concentration of 15.5 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a numerical PSF model 71154a - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 22.5 micron^2/s - 60 second simulation using a numerical PSF model ad926d - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 5.625 micron^2/s - 60 second simulation using a numerical PSF model 1ab235 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s - 180 second simulation using a numerical PSF model d00978 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 5.625 micron^2/s - 180 second simulation using a numerical PSF model   2469bb - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a Gaussian PSF model  4be121 - 15 molecules at a concentration of 31 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a Gaussian PSF model a7088f - 15 molecules at a concentration of 15.5 pM, with a diffusion coefficient of 90 micron^2/s - 60 second simulation using a Gaussian PSF model 023983 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 22.5 micron^2/s - 60 second simulation using a Gaussian PSF model 653f61 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 5.625 micron^2/s - 60 second simulation using a Gaussian PSF model 4f06ee - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s - 180 second simulation using a Gaussian PSF model dec32c - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 5.625 micron^2/s - 180 second simulation using a Gaussian PSF model   85b0a1 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s , 10 of which belong to a sub-population with a mean FRET efficiency of 0.75, and the leftover 5 belong to another sub-populations with a mean FRET efficiency of 0.50 - 60 second simulation using a Numerical PSF model 964ef3 - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s , 10 of which belong to a sub-population with a mean FRET efficiency of 0.75, and the leftover 5 belong to another sub-populations with a mean FRET efficiency of 0.50 - 180 second simulation using a Numerical PSF model   f28f6e - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s , 10 of which belong to a sub-population with a mean FRET efficiency of 0.75, and the leftover 5 belong to another sub-populations with a mean FRET efficiency of 0.50 - 60 second simulation using a Gaussian PSF model c311dd - 15 molecules at a concentration of 62 pM, with a diffusion coefficient of 90 micron^2/s , 10 of which belong to a sub-population with a mean FRET efficiency of 0.75, and the leftover 5 belong to another sub-populations with a mean FRET efficiency of 0.50 - 180 second simulation using a Gaussian PSF model
创建时间:
2020-01-24
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