Flow cytometry raw data involving in the article "Neuro-immune–related gene signatures define molecular subtypes and prognostic score in bladder cancer with SERPINE2 as a potential therapeutic target"

Purpose This experiment aimed to determine the effect of SERPINE2 gene knockdown on apoptosis in T24 human bladder cancer cells using the Annexin V-FITC/PI dual staining method. Experimental Design Groups: Control (Vector), shRNA3, Cell line: T24 (human bladder carcinoma), Method: Flow cytometry (FITC/PI dual channel), Replicates: 3 biological repeats MaterialsItemBrandCatalog No.NoteDMEM High Glucose MediumGibco11965-092with 10% FBS1× PBSYEASEN41403ES76pre-cooledTrypsin-EDTA SolutionGibco25200-0720.25%Annexin V-FITC Apoptosis Detection KitBeyotimeC1062Lper manufacturer’s protocolInstrumentation Flow cytometer: BD FACSCanto II (BD Biosciences, USA) Excitation laser: 488 nm Channels: FITC: Ex/Em = 488/530 nm, PI: Ex/Em = 488/615 nm Software: FlowJo v10.8 Data Description Format: .FCS Each group includes 3 replicates Example file names: T24-NC_1.fcs T24-shRNA3_1.fcs Summary of Results Knockdown of SERPINE2 significantly increased the apoptosis rate of T24 cells compared with the control group (P