The E41Q mutant of the amidase from Nesterenkonia sp. AN1 showing covalent addition of the acetamide moiety of fluoroacetamide at the active site cysteine

The E41Q mutant of the amidase from Nesterenkonia sp. AN1 showing covalent addition of the acetamide moiety of fluoroacetamide at the active site cysteine Descriptor: 2-fluoroacetamide, ACETAMIDE, Amidase Authors: Kimani, S.W, Sewell, B.T. Deposit date: 2013-01-30 Release date: 2014-02-12 Last modified: 2024-11-20 Method: X-RAY DIFFRACTION (1.92 Å) Cite: Covalent modifications of the active site cysteine occur as a result of mutating the glutamate of the catalytic triad in the amidase from Nesterenkonia sp. To be Published

来自涅斯捷连科氏菌（Nesterenkonia）sp. AN1的酰胺酶（amidase）E41Q突变体，其活性位点半胱氨酸（active site cysteine）上发生了氟乙酰胺（fluoroacetamide）的乙酰胺（acetamide）片段的共价加成反应。描述符：2-氟乙酰胺、乙酰胺、酰胺酶。作者：Kimani, S.W.、Sewell, B.T.。存储日期：2013-01-30。发布日期：2014-02-12。最后修改日期：2024-11-20。实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率1.92 Å。引用说明：涅斯捷连科氏菌属酰胺酶催化三联体（catalytic triad）中的谷氨酸残基发生突变后，会导致活性位点半胱氨酸发生共价修饰，该研究待发表。