Transcriptomic changes in SUDHL6 cells upon treatment with S63845 in the presence or abscence of active caspases

We identified a novel form of caspase-independent cell death (CICD) induced by BH3-mimetics. To characterize the transcriptional changes during CICD and compare these to apoptotic changes, we performed RNA Seq analysis on SUDHL6 lymphoma cells treated with the MCL1 inhibitor S63845 in the presence or abscence of the broad spectrum caspase inhibitor zVAD.fmk. Overall design: RNA Seq analaysis was performed on biological duplicates (sample 1 and sample 2). "Control" are untreated SUDHL6 cells, "S63845" were treated with 300 nM of S63845 for 8h, and "S63845+zVAD" were treated with 300 nM S63845 and 20 uM zVAD.fmk for 8 hours.