Tensile properties of bone collagen in the human proximal femur (FEMCOL)

Summary This dataset comprises the following imaging, compositional and mechanical data for eighty human bone sections of the proximal, lateral part of the femoral diaphysis: Micro-CT (uCT) Tensile mechanical test Raman spectroscopy Gravimetric analysis Sample Information The dataset consists of eighty human bone samples from the proximal, lateral diaphyseal region of the femur originating from 24 female and 19 male donors aged 57 to 96 years at time of death. For each sample, information about the laterality (left/right bone), age and sex of the donor is provided. All specimens were obtained by the Division of Anatomy of the Medical University of Vienna, Austria, with the informed consent of the donors. The file SampleList.csv contains the following information. Sample ID Sample ID, which allows the data in this repository to be linked to other projects using the same femora. For further information, please refer to the projects listed under related works. uCT ID Sample ID for uCT scans age Donor age at time of death in years sex Donor sex, F: female, M: male side Laterality of bone, L: left, R: right Missing values are denoted with NA. Methods A detailed description of the methods related to the presented data can be found in the associated publication:Bracher, Stefan, et al. "Bone collagen tensile properties of the aging human proximal femur." Bone reports 21 (2024): 101773. Data Micro-CT Micro-CT scans (uCT 100, SCANCO Medical AG, Brüttisellen, Switzerland) were obtained at a resolution of 7.4 um and are available in .ISQ format. Due to the large file size, the scans are not stored on this zenodo repository, but are available on request. For additional information, please contact [e-mail]. Tensile Mechanical Tests Force displacement data of uniaxial tensile tests is available for mineralized and demineralized bone samples. Native samples were loaded for eight preconditioning cycles up to 0.25% strain in tension. Demineralized samples were preconditioned for eight cycles between 2-3 % strain and subsequently loaded to failure. The force displacement data is available in .csv format and contains the following information. The sample ID is included in the file name. Time Time in s RL_20000A-100SS_load_cel Reaction force in N measured by the load cell (RL20000A-100 SS, Rice Lake Weighing Systems, Wisconsin, United States) Xtens_longitudinal_mm Displacement in mm measured by the extensometer (laserXtens 1-15 HP, ZwickRoell GmbH & Co. KG, Ulm, Germany) Raman Spectroscopy Raman spectroscopy was performed to determine mineral to matrix ratio, crystallinity, collagen disorder, matrix maturity, and relative proteoglycan content. The results are available in .csv format. Sample ID Sample ID, which allows the data in this repository to be linked to other projects using the same femora. For further information, please refer to the projects listed under related works. Mineral to Matrix Ratio v2/a3 / - Ratio of the secondary phosphate band integral area (v2PO4, 410–460 cm−1) over the amide III band integral area (1215–1300 cm−1), unitless Mineral to Matrix Ratio v1/a1 / - Ratio of the primary phosphate band integral area (v1PO4, 910–995 cm−1) over the amide I band integral area (1590–1730 cm−1), unitless Crystallinity / - Inverse value of the primary phosphate (v1PO4, 910–995 cm−1) full width at half maximum, unitless Collagen dis/order / - Intensity ratio of the amide I sub bands I∼1670/I∼1640, unitless Matrix maturity / - Intensity ratio of the amide I sub-bands I∼1660/I∼1683, unitless Relative Proteoglycan Content / - Ratio of Glycosaminoglycan band integral area (1360–1390 cm−1) over amide III band integral area (1215–1300 cm−1), unitless Missing values are denoted with NA. Gravimetry The sample’s mineral, organic and water weight as well as the corresponding weight fractions were determined by gravimetric analysis. The results are available in .csv format. Sample ID Sample ID, which allows the data in this repository to be linked to other projects using the same femora. For further information, please refer to the projects listed under related works. wet weight / g Sample weight in hydrated state (48h immersion in dH2O) after centrifugation at 15 relative centrifugal force for 5 min (5415 R, Eppendorf AG, Hamburg, Germany) expressed in g. dry weight / g Sample weight determined after drying the sample for 48 h at 40 °C in an ashing furnace (LVT 3/11, Nabertherm GmbH, Lilienthal/Bremen, Germany) expressed in g. ash weight / g Sample weight after ashing the sample for 12 h at 800 °C in an ashing furnace (LVT 3/11, Nabertherm GmbH, Lilienthal/Bremen, Germany) expressed in g. immersed weight / g Sample weight under immersion in distilled water for 48 h, expressed in g. density / g/cm^3 Bone density determined by Archimedes’ principle where is the wet weight of the sample, is the immersed weight of the sample and is the density of distilled water at 23°C (0.99754 g/cm3). Bone density is expressed in g/cm3. organic weight / g Weight of the organic bone portion expressed in g and calculated as mineral weight / g Weight of the mineral portion in the sample expressed in g. Corresponds to ash weight. water weight / g Weight of the water portion in the hydrated bone sample expressed in g and calculated as weight fraction of mineral phase / - Relative weight of mineral phase to overall bone mass, calculated as , unitless weight fraction of organic phase / - Relative weight of organic phase to overall bone mass, calculated as , unitless weight fraction of water phase / - Relative weight of the water phase to the overall bone mass, calculated as Data Access Access to this repository is restricted for data protection and privacy reasons. To request access to this dataset, please submit a request through Zenodo and provide a brief description of the intended use of the data. Access will be granted only after completion and signing of a Data Transfer and Use Agreement. Contact Philippe Zysset ([e-mail]) for further questions. Related Work Associated Publication Bracher, Stefan, et al. "Bone collagen tensile properties of the aging human proximal femur." Bone reports 21 (2024): 101773. Other Projects Using the Same Femur Samples Simon, M., Gerber, G.& Zysset, P. (2026). FEXHIS dataset for automatic segmentation of cortical bone microstructure [Dataset]. Zenodo. https://doi.org/10.5281/zenodo.21666670 Voumard, B., Dudle, A., Gugler, Y., Gerber, G.& Zysset, P. (2026). Multiscale imaging and mechanical testing dataset of human proximal femora (FEMHALS) [Dataset]. Zenodo. https://doi.org/10.5281/zenodo.21494458