Additional file 3 of The intrinsically disordered region of GCE protein adopts a more fixed structure by interacting with the LBD of the nuclear receptor FTZ-F1
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Additional file 3: Figure S3. LBD FTZ-F1 purification. A) Preparative SEC performed on the Superdex 75 10/300 GL column. Fractions co’ntaining purified LBD FTZ-F1 protein are marked with the grey color. B) Commassie Brilliant Blue R 250-stained SDS-PAGE analysis of the LBD FTZ-F1 samples. Lane 1, the bacterial protein fraction; lane 2, the soluble protein fraction; lane 3, the fraction of proteins not bound to the Ni2+-NTA resin; lane 4, combined elution fractions; lane 5, LBD FTZ-F1 purified with SEC; lane 6, molecular mass standards.
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2021-04-16
