Optimization of fermentation parameters to improve hydrogen production from citrus agroindustry waste. Metagenome

Energy recovery with the production of hydrogen through dark fermentation using agroindustrial wastes has been widely evaluated, however, the adjustment of operational conditions is still an obstacle to conducting a viable process with high H2 production. For this purpose, a Rotational Central Composite Design (DCCR) and response surface methodology (RSM) were applied to obtain a model for optimizing H2 production, based on the citrus peel wastes concentration (CPW) (9.9 a 35.1 gSTV L-1), wastewater concentration (CPWW) (0.3 a 8.7 gCOD L-1) and CaCO3 concentration (0.3 a 7.3 g L-1).