Additional file 1 of Third generation cephalosporin-resistant Klebsiella pneumoniae thriving in patients and in wastewater: what do they have in common?

Additional file 1 : Table S1. 3rd generation cephalosporin-resistant K. pneumoniae isolates characterized based on β-lactam and carbapenem encoding genes, antimicrobial resisitance phenotype, plasmid number, size and replicon types, conjugation properties and biofilm formation capacity. A total of 25 isolates were obtained from wastewater, 30 from patients and 4 from clinical settings. Table S2. Antimicrobial resistance phenotype and genotype transferred to transconjugants by the conjugative isolates from the 59 isolates of K. pneumoniae resistant to 3rd generation cephalosporins. + indicates detection of the trait, − indicates not detection of the trait, S, I and R indicate susceptible, intermediate and resistant to the antibiotic, and NA indicates not assessed because the trait was not detected in the donor cell. Table S3. Clusters obtained based on the pheno- and genotypic characteristics of the 3rd generation cephalosporin-resistant K. pneumoniae isolates and dominant characteristics observed in each cluster. Table S4. 3rd generation cephalosporin-resistant K. pneumoniae isolates genomes used for comparative genomic analysis. Table S5. Genes searched related to clinically-relevant properties on selected 3rd generation cephalosporin-resistant K. pneumoniae genomes. 1 indicates the detection of the gene and 0 indicates the not detection of the gene. For each gene it is indicated the allele that was detected, the number of genomes in which it was detected, the length of the gene and of the query, the number of different nucleotides in the query sequence and the number of gaps. The accession number and the database from which each gene allele was downloaded is also indicated. Table S6. Primer sequences and PCR amplification conditions used in this study.