Richard Allen (2010) CIL:9911, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

An image of the contractile vacuole and the spongiome decorated tubules showing V-type proton pumps extending from the cytosolic side of the tubules; also detail of the micronucleus. TEM taken on 6/10/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. Bar = 0.25µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:7305) is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

本数据集呈现了一幅收缩空泡及其装饰性管状结构中V型质子泵从管状结构的细胞质侧延伸出的图像；亦包含了微核的详细视图。该透射电子显微镜（TEM）图像由R. Allen于1969年6月10日使用Philips 300型仪器，在60kV的电压下拍摄。负片放大倍数为14,800倍。标尺长度为0.25微米。负片的打印件经扫描并在Photoshop中处理。此图像最适合进行定性分析。高分辨率图像（CIL:7305）可用于定量分析。样本采用标准戊二醛固定法，随后进行四氧化锇处理，经酒精脱水并浸渍于环氧树脂中。微切片厚度约为75纳米。更多详细信息请访问（http://www5.pbrc.hawaii.edu/allen/）。