Systematic tethering approach assigns function to RNA binding proteins and identifies UBAP2L as an antagonist to FMRP-mediated translation repression (Neuron_polysome_RNA-seq). Systematic tethering approach assigns function to RNA binding proteins and identifies UBAP2L as an antagonist to FMRP-mediated translation repression (Neuron_polysome_RNA-seq)

In these FMRP knockout lines we then depleted UBAP2L by lentiviral delivery of two different shRNAs, with a non-targeting shRNA serving as a control. Then, differentiated all lines to cortical neurons using a directed protocol (Nehme et al., 2018), which is based on a modified version of that of (Zhang et al., 2013)). We then performed polysome profiling by measuring the ratios of transcript levels in polysome factions over inputs Overall design: RNAseq