ion intensity (merged)

Intra- and extra- cellular non-targeted metabolomes of primary human monocyte-derived macrophages. Table contains a list of ions, m/z ratio, putative annotation based on m/z ratio, module membership and log intensities.Experimental protocol: PBMCs were isolated from leukoreduction system chambers and buffy coats obtained with written informed consent from healthy anonymous blood donors in the UK and The Netherlands, respectively. CD14⁺ monocytes were isolated using CD14 magnetic beads and MACS columns (Miltenyi). The subsequent monocytes were then differentiated into macrophages over 6 days using 50 ng/mL human M-CSF (Miltenyi) and cultured in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 10% heat-inactivated fetal bovine serum, 1% Penicillin/Streptomycin solution, and 1% L-glutamine solution (Gibco). On day 6, the medium was replaced with culture medium without M-CSF and supplemented with the following stimuli: no stimulus, 10 ng/mL LPS (Sigma, E. coli E55:O5), 10 ng/mL LPS plus 50 ng/mL IFN-γ (R&amp;D), 50ng/mL IFN-γ (R&amp;D), 50 ng/mL IL-4 (PeproTech), 50 ng/mL IL-10 (R&amp;D), 100 nM dexamethasone (Sigma) for 24 hours.<br>Annotation:M0: unstimulated macrophages (24hr medium) M-LPS: 24hr LPS, M-LPSIFNγ: 24hr LPS and IFN-γ, M-IFNγ: 24hr IFN-γ, M-IL4: 24hr IL-4,M-IL10: 24hr IL-10, M-dex: 24hr dexamethasonenumbers at the end of the sample ID represent donor numbers.associated preprint: https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4647290