Telomeres of chromosome arms other than cXIIr barely missegregated after a cdc14-1 release.

aStrains FM565 (cdc14-1 tel V-L:tetO tel V-R:lacO tetR-YFP LacI-CFP) and FM573 (cdc14-1 tel XIV-L:lacO tel XIV-R:tetO tetR-YFP LacI-CFP) were arrested in telophase (37°C) for 3 h and then released to 25°C. Samples were then taken, micrographed after DAPI staining and scored for telomere missegregation (binucleated foursomes only).bStrains FM567 (cdc15-2 tel V-L:tetO tel V-R:lacO tetR-YFP LacI-CFP) and FM574 (cdc15-2 tel XIV-L:lacO tel XIV-R:tetO tetR-YFP LacI-CFP) were arrested in telophase (34°C) for 3 h. Samples were then taken, micrographed after DAPI staining and scored for telomere missegregation (binucleated dumbbells only).cStatistical significance of cross-comparison between cdc14-1 release (2 h) and cdc15-2 block. Student's T test.