Comparison of gene expression between islets from NOD-Rag and NOD-Idd22 mice

NOD-Idd22 mice are congenic mice of NOD background with a piece of chromosome 8 being substituted with ALR genetic material. These mice are resistant to spontaneous autoimmune diabetes as well as chemically induced in vivo islet beta cell destructions. The goal of this project is to come pare gene expressions in islets between NOD-Idd22 and NOD mice. NOD-Rag1 was used instead of NOD to avoid lymphocyte infiltrtation in isltes. Each sample of RNA was extracted from about 200 islets that were isoleated and pooled from 2 mice. Four replicates from NOD-Idd22 and 3 replicates from NOD.Rag1 were analyzed.