Sheep Heart Transcriptomes following pulmonary artery banding (PAB) and right sided heart failure

Sheep were studied using pulmonary artery banding (PAB) and various regions of the heart were dissected. Three experimental studies were performed to address 8 weeks after PAB vs controls, 8 weeks of PAB followed by reversal surgery and 8 more week of recovery, and male vs female sheep after 12 weeks of PAB. RNA was extracted using TRIzol and ribosomal reduced paired end sequencing was performed using Illumina.