Data from: Experimental and statistical reevaluation provides no evidence for Drosophila courtship song rhythms

From 1980 to 1992, a series of influential papers reported on the
discovery, genetics, and evolution of a periodic cycling of the interval
between Drosophila male courtship song pulses. The molecular mechanisms
underlying this periodicity were never described. To reinitiate
investigation of this phenomenon, we previously performed automated
segmentation of songs but failed to detect the proposed rhythm [Arthur BJ,
et al. (2013) BMC Biol 11:11; Stern DL (2014) BMC Biol 12:38]. Kyriacou et
al. [Kyriacou CP, et al. (2017) Proc Natl Acad Sci USA 114:1970–1975]
report that we failed to detect song rhythms because (i) our flies did not
sing enough and (ii) our segmenter did not identify many of the song
pulses. Kyriacou et al. manually annotated a subset of our recordings and
reported that two strains displayed rhythms with genotype-specific
periodicity, in agreement with their original reports. We cannot replicate
this finding and show that the manually annotated data, the original
automatically segmented data, and a new dataset provide no evidence for
either the existence of song rhythms or song periodicity differences
between genotypes. Furthermore, we have reexamined our methods and
analysis and find that our automated segmentation method was not biased to
prevent detection of putative song periodicity. We conclude that there is
no evidence for the existence of Drosophila courtship song rhythms.