RNA Immunoprecipitation in macrophages using NLRC4 and Caspase11 and high-throughput sequencing of isolated lncRNA

At the epigenetic level, lncRNAs play important roles in cell differentiation and function. We identified novel long-stranded non-coding RNA (GM1082) in BMDMs, which may play an important role in macrophage differentiation and function.To confirm the interaction between LNCRNA and NLRC4 and Caspase11, we performed RNA immunoprecipitation using NLRC4 and Caspase11 antibodies, and high-throughput sequencing of the obtained RNA. Overall design: Mouse bone marrow cells were induced in vitro by adding 20ng/ml of M-csf in DMEM medium.After 6 days of M-CSF induction, BMDMs were exposed to flagellin.Cell lysates were incubated with normal rabbit IgG, NLRC4 and caspase11 antibody.RNAs were isolated from the Protein–RNA complexes with phenol-chloroform and subjected to library construction.