Research on the circular RNA bioinformatics in patients with acute myocardial infarction

To further development of our gene expression approach to biodosimetry, We through the detection of circular RNA (circRNA) using expression profiling chips, we searched for circRNAs related to acute myocardial infarction (AMI) and explored their relationship and possible mechanisms with AMI. The study subjects included 3 AMI patients and 3 controls, and circRNA expression profiling analysis was performed using a microarray gene chip to identify circRNAs with large differences in expression between groups and to construct a circRNA-microRNA (circRNA-miRNA) network. The total RNA from the each patient's plasma was extracted using TRIzol reagent according to the manufacturer's instructions. The purity and concentration of the RNA were determined from a 260/280 reading by using a spectrophotometer. RNA integrity was determined with a lab-on-a-chip kit using an RNA 6000 nano chip and Bioanalyzer 2100 to assess RNA quality comprehensively. Fluorescent dye Cy3 dCTP-labeled cDNA was prepared using Eberwine linear RNA amplification and enzymatic reaction. Data normalization and difference analysis were performed on the circRNA array data using GeneSpring software V13.0.