Development of A. fumigatus morphotypes interacting with iDCs in co-cultures over 12 h in RPMI medium (supplemented with 10% FCS).
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The counts derived from light microscopic observation of at least ten fields of view containing 200 cells at each time point; the total cell (fungal element) numbers included phagocytosed conidia. The numbers are mean ± standard error from three replicate experiments. Swollen conidia were distinguished by being large and appearing to having a thinner cell wall due to loss of the rodlet layer. Hyphae were distinguished from germ tubes by being longer in length >50 µm.
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2015-12-02



