Agarose gel images from the PCR genotyping of the F2 crosses (A) SS12S6 × abp1-c1, (B) SS12S6 × abp1-TD1, (C) abp1-AS × abp1-c1, (D) abp1-AS × abp1-TD1, (E) SS12K9 × abp1-c1 and (F) SS12K9 ×abp1-TD1 (Figure 3)

All crosses were genotyped for the presence of the alcR transcriptional regulator (first row of the gel images) which is an integral part of the ethanol-inducible cassette in abp1 knock-down lines. The presence of point mutation in abp1-c1 crosses was genotyped by restriction analysis of ABP1 PCR product as described in Gao et al. (2015) (second row of the gel images of abp1-c1 crosses). The presence of the T-DNA insertion in abp1-TD1 crosses was genotyped according to Gao et al. (2015) (second and third row of gel images of abp1-TD1 crosses). GeneRuler DNA ladder mix #0331 (Thermo Scientific) was used as a fragment size standard to determine the approximate size of DNA fragments. Fragment sizes of 1000 bp and 500 bp are indicated.