Figure 5_plot binding curves
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BMK-DKO cells expressing CBcl-W, transfected with VBH3 proteins treated with BH3 mimetics. This folder contains each binding curve plotted in STEP 10 of qF3 analysis. Filename includes information: \"Well ID_Donor Fusion Protein_Transfectant_Treatment_Treatment concentration\". Where a decimal in concentration is replaced with a dash in the filename. ie. 1.25uM is 1-25uM. The Filename of the file corresponds to the data plotted in Red. Positive and Negative controls (VBH3 and VBH3-4E) are plotted on each graph for comparison in Blue and Grey, respectively. If the data could be fit, then fit is displayed on the graph. Data displayed in Figure 5a-d was generated from combining results of 3 screens: 1) mim1_BclW_screen {Rep1_20190212, Rep2_20190213, Rep3_20190214, Rep4_20190215} 2) mim2_BclW_screen {Rep1_20190403, Rep2_20190404, Rep3_20190405, Rep4_20190406} 3) XL2W_screen {Rep1_20191108, Rep2_20191109, Rep3_20191110, Rep4_20191111} Figure 5f-g was generated from BH3 swap screen {Rep1_20200821, Rep2_20200827, Rep3_20200902, Rep4_20200904} - see, \"Figure 5\" Dataset in dataverse for associated .CSV files for each binding curve.
创建时间:
2023-12-28



