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TopFD - A Software Tool for Top-Down Mass Spectrometry Feature Detection

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NIAID Data Ecosystem2026-03-14 收录
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https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=3f0af3d60c124373aa776b518a2171e6
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The two data sets were generated from SW480 and SW620 colorectal cancer (CRC) cells. Proteoforms extracted from the sample were analyzed using a Thermo Q-Exactive HF mass spectrometer coupled with a 105-minute nanoRPLC separation system. The top 5 precursor ions in each MS1 spectrum were selected from an isolation window of 4 m/z for MS/MS analysis using higher-energy collisional dissociation (HCD). Both MS1 and MS/MS spectra were acquired at a resolution of 120,000 (at 200 m/z). Three technical replicates were obtained.
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2022-10-07
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