Single-nucleus Transcriptomics Reveals Cardiac Cell Type-specific Diversification in Metabolic Disease Transgenic Pigs

Cardiac injury triggered by early metabolic disease is poorly investigated due to the complexity of pathophysiology. We comprehensively reveal the cellular landscape of healthy and metabolically disturbed heart through single-nucleus RNA sequencing, using the metabolic disease-susceptible transgenic pig model as material. Overall design: We performed single-nucleus RNA sequencing on left ventricular myocardium from both triple-transgenic pig(PNPLA3I148M-GIPRdn-hIAPP) (TG) and wild-type pig (WT).