Transcriptome analysis of Traf6 function in early zebrafish embryogenesis

In order to study Traf6 function during early zebrafish embryogenesis, transcriptome expression profiling was performed at 30% epiboly using a morpholino based knock-down approach. Two-factorial design with the factors 'Treatment' (5 levels:'Phenol Red', 'Standard Control', 'Mismatch', 'Morpholino1' and Morpholino 2') and 'Dose' (3 levels: 'Low', 'Middel' and 'High') with the factor 'Dose' only being applicable to the morpholino injections and not to the 'Phenol Red' control.