RNA-seq of B. olearacea x X. campestris pathosystems. In vivo transcriptome profiling of Xanthomonas campestris pv. campestris strains with contrasting virulence

Black rot disease caused by Xanthomonas campestris pv. campestris (Xcc), is the leading bacterial disease of Brassicaceae, affecting crops worldwide. Studies on the molecular mechanisms interaction of Xcc - B. oleracea have identified several virulence genes, mostly inferred using in vitro approaches, thus resulting in a knowledge gap concerning the in vivo behavior of this pathogen during pathogenesis. Within this framework, the pathogenicity and virulence of a set of Xcc strains was characterized, and the in vivo transcriptomes of strains in the extremes of the virulence spectrum were profiled, using RNA-Seq, bringing new insights on the molecular mechanisms of host-pathogen interaction.