Gene expression profile of endothelial cell cultures exposed to uremic serum from Chronic Kidney Disease patients: role of MAPK signaling pathway

Transcriptome analysis of human coronary arterial endothelial cells (HCAEC) primary cultures exposed to uremic serum from patients with stage 4-5 cardiovascular diseases (CKD) without myocardial infarction (USI) and stage 4-5 CKD with myocardial infarction (UCI). Gene expression profile of human coronary arterial endothelial cells (HCAEC) primary cultures exposed to uremic serum from of patients with chronic kidney disease (CKD) that explore which pathways are involved in in the development of cardiovascular disease. The metabolic pathway significantly represented was the MAPK signaling pathway, suggesting that the effects of uremic toxins present in patients with CDK, on the primary cultures of HCAEC provides a more informative model for the study of molecular mechanisms that lead to the development of endothelial dysfunction secondary to CKD. We analyzed RNA of human coronary arterial endothelial cells (HCAEC) primarycultures supplemented with 20% uremic serum from two groups of patients, group 1: patients with stage 4-5 Chronic kidney disease (CKD) without myocardial infarction (USI) and group 2: stage 4-5 CKD with myocardial infarction (UCI). We used Affymetrix GeneChip® microarray and the LIMMA package (Linear Models for Microarray Data) of the Bioconductor software17 was implemented to identify relevant DEGs between the two groups of Uremic patients.