L1000 Connectivity Map perturbational profiles from Broad Institute LINCS Center for Transcriptomics LINCS PHASE *II* (n=354,123; updated March 30, 2017) (Level 5 data)

The Library of Integrated Cellular Signatures (LINCS) is an NIH program which funds the generation of perturbational profiles across multiple cell and perturbation types, as well as read-outs, at a massive scale. The LINCS Center for Transcriptomics at the Broad Institute uses the L1000 high-throughput gene-expression assay to build a Connectivity Map which seeks to enable the discovery of functional connections between drugs, genes and diseases through analysis of patterns induced by common gene-expression changes. This is Level 5 data: GSE70138_Broad_LINCS_Level5_COMPZ_n118050x12328_2017-03-06.gctx.gz Series GSE70138 L1000 data is provided at five levels of the data processing pipeline: Level 1: Raw unprocessed flow cytometry data from Luminex (LXB) Level 2: Gene expression values per 1000 genes after deconvolution (GEX) Level 3: Quantile-normalized gene expression profiles of landmark genes and imputed transcripts (Q2NORM or INF) Level 4: Gene signatures computed using z-scores rela

整合细胞信号库（LINCS）是美国国家卫生研究院（NIH）资助的一个项目，旨在大规模生成多种细胞和扰动类型的扰动谱以及读数。位于Broad研究所的LINCS转录组中心采用L1000高通量基因表达检测技术构建连通性图谱，旨在通过分析由共同基因表达变化诱导的模式，实现药物、基因与疾病之间功能联系的发现。此数据集属于第5级数据：GSE70138_Broad_LINCS_Level5_COMPZ_n118050x12328_2017-03-06.gctx.gz。L1000数据在数据处理流程的五个级别提供：第1级：来自Luminex（LXB）的原始未经处理的流式细胞术数据；第2级：去卷积后每1000个基因的基因表达值（GEX）；第3级：里程碑基因和推断转录本的分位数归一化基因表达轮廓（Q2NORM或INF）；第4级：使用z分数计算的基因特征。