Minimal PutA proline dehydrogenase domain (design #2) covalently inactivated by (S)-but-3-yn-2-ylglycine

Minimal PutA proline dehydrogenase domain (design #2) covalently inactivated by (S)-but-3-yn-2-ylglycine Descriptor: Bifunctional protein PutA, FORMIC ACID, {[(3E)-4-{10-[(2S,3S,4R)-5-{[(R)-{[(R)-{[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-2,3,4-trihydroxypentyl]-7,8-dimethyl-2,4-dioxo-2,3,4,10-tetrahydrobenzo[g]pteridin-5(1H)-yl}but-3-en-2-ylidene]amino}acetic acid (non-preferred name) Authors: Tanner, J.J, Meeks, K.R, Ji, J. Deposit date: 2024-08-16 Release date: 2025-02-05 Last modified: 2025-02-19 Method: X-RAY DIFFRACTION (1.68 Å) Cite: Biochemical, structural, and cellular characterization of S-but-3-yn-2-ylglycine as a mechanism-based covalent inactivator of the flavoenzyme proline dehydrogenase. Arch.Biochem.Biophys., 765, 2025最小化PutA脯氨酸脱氢酶（proline dehydrogenase）结构域（设计编号2）经(S)-丁-3-炔-2-基甘氨酸共价灭活。描述：双功能蛋白PutA、甲酸、{[(3E)-4-{10-[(2S,3S,4R)-5-{[(R)-{[(R)-{[(2R,3S,4R,5R)-5-(6-氨基-9H-嘌呤-9-基)-3,4-二羟基四氢呋喃-2-基]甲氧基}(羟基)磷酰基]氧基}(羟基)磷酰基]氧基}-2,3,4-三羟基戊基]-7,8-二甲基-2,4-二氧代-2,3,4,10-四氢苯并[g]蝶啶-5(1H)-基}丁-3-烯-2-亚基]氨基}乙酸（非首选名称）。作者：Tanner J.J.、Meeks K.R.、Ji J.。提交日期：2024-08-16。发布日期：2025-02-05。最后修改日期：2025-02-19。实验方法：X射线衍射（1.68埃）。引用文献：《S-丁-3-炔-2-基甘氨酸作为黄素酶（flavoenzyme）脯氨酸脱氢酶的机制型共价灭活剂的生化、结构与细胞表征》，《Archives of Biochemistry and Biophysics》，765卷，2025年。