Transfer of Fe-S clusters to SDHB

After translation in cytosol and translocation to the mitochondrial inner membrane SDH subunits C and D (SDHC, SDHD), together with the heme b cofactor, bind to the assembled soluble SDHA:SDHB construct to form the fully functional SDH complex in the mitochondrial membrane. Although a full structure of the porcine complex exists (PDB 1ZOY) no details are known about the last steps of the assembly. However, successful assembly depends on the presence of both the SDHC heme b cofactor and the C-terminal tail of the SDHA subunit. (Lemarie & Grimm, 2009; Kim et al., 2012; reviewed in Van Vranken et al., 2014; Signes & Fernandez-Vizarra, 2018).

在细胞质翻译及向线粒体内膜转移后，SDH 亚基 C 和 D（SDHC，SDHD）与血红素 b 辅因子相结合，并与已组装的溶解性 SDHA:SDHB 结构体结合，从而在线粒体内膜中形成功能完备的 SDH 复合物。尽管已存在猪类复合物的完整结构（PDB 1ZOY），但对于组装的最后步骤仍知之甚少。然而，成功的组装依赖于 SDHC 血红素 b 辅因子和 SDHA 亚基的 C 端尾部的存在。（参见 Lemarie & Grimm，2009；Kim et al.，2012；综述于 Van Vranken et al.，2014；Signes & Fernandez-Vizarra，2018）。