Single cell RNA-sequencing of normal and fibrotic lungs in mouse and human

We performed scRNA-seq of cells obtained with a protocol designed to capture all of the collagen-producing cells from normal and fibrotic mouse and human lungs. We induced lung fibrosis by treating two Col1a1-GFP reporter mice with bleomycin, and harvested lungs on day 14 after treatment. Two untreated Col1a1-GFP mice were used as control. After tissue dissociation, GFP+ cells and GFP- cells were sorted, and their single cell libraries were generated using 10x Genomics 3' v2 system. For human samples, we sorted lineage (CD31, CD45, EPCAM, CD235a)- cells and all lung cells (CD235a-) from three normal, three IPF, and two scleroderma lungs.