five

Quantitative monitoring of environmental DNA using high-throughput sequencing

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DataONE2022-09-20 更新2025-09-20 收录
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The goal of this project is to monitor the dynamics of environmental DNA (e.g., fish DNA found in a water sample) quantitatively. By adding internal standard DNA, a standard curve (i.e., the relationship between the number of DNA copy and sequence read) will be drawn. Based on the standard curve, sequence reads of unknown DNA (non-standard DNA) will be converted to the number of DNA copy. The environmental DNA metabarcoding combined with internal standard DNA will enable quantitative monitoring of multispecies environmental DNA.
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2025-09-16
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