Dynamic Isoelectric Focusing for Proteomics

Dynamic isoelectric focusing is a new technique that is
related to capillary isoelectric focusing but uses additional
high-voltage power supplies to provide control over the
shape of the electric field within the capillary. Manipulation of the electric field changes the pH gradient, enabling
both the location and width of the focused protein bands
to be controlled. The proteins can be migrated to a
designated sampling point while remaining focused, where
they can be collected for further analysis. This ability to
collect and isolate the protein bands while maintaining a
high peak capacity demonstrates that dynamic isoelectric
focusing has great potential as a first dimension in a
multidimensional separation system. Dynamic isoelectric
focusing can achieve a peak capacity of over 1000, as
shown by both mass spectrometry analysis and direct
imaging.