Single cell RNA-seq for PBMC from rheumatoid arthritis patient

The transcriptome of PBMC from rheumatoid arthritis patient hasn't been compenhensively profiled, and heterogeneous characteristics of blood monocytes in RA patients are much unknown. We performed the single cell transcriptomic analysis of PBMC from rheumatoid arthritis (RA) patient, and monocyte populations were extracted during the analysis. CD127 expression associated expression pattern of inflammatory genes was identified in RA patient's blood monocytes. PBMC were isolated from peripheral blood of RA patient by Ficoll gradients, and isolated PBMC were frozen for preservation and sent out for scRNA-seq library construction by using 10x genomics platform.