DataSheet_1_Taxonomy and phylogeny of Pseudovorticella ciliates (Ciliophora, Peritrichia): Two new and one rare species from the coastal waters of China.zip

Peritrich ciliates are a species-rich group of sessile unicellular eukaryotes, which can be found in various aquatic habitats from all over the world. It is well accepted that there are still many ciliates to be uncovered. During a survey on ciliate biodiversity in the coastal waters of China, three solitary peritrich species were identified as members of the genus Pseudovorticella Foissner & Schiffmann, 1975, including two new species and a rare one. Pseudovorticella zhejiangensis sp. n. differs from its congeners mainly by having a conical-shaped zooid, conspicuous pellicular blisters, one ventral and one dorsal contractile vacuoles, and an infundibular polykinety 3 with three rows of nearly equal length but different beginning positions. Pseudovorticella dalianensis sp. n. can be defined mainly by an obovoid-shaped zooid, one ventral contractile vacuole, and a three-rowed infundibular polykinety 3 with the middle row being the longest. The rare species, Pseudovorticella verrucosa (Dons, 1915) Sun et al., 2009, was redescribed. The small subunit (SSU) rDNA sequences of these three species were sequenced for the first time, the phylogeny of Pseudovorticella species was analyzed, and the results verified the non-monophyly of this genus. This study demonstrates that the morphologic and gene barcoding data are the optimum combination to disclose the biodiversity of ciliates.

多毛纲纤毛虫是一群物种丰富的固着性单细胞真核生物，它们遍布全球各种水生栖息地。普遍认为，仍有大量纤毛虫未被揭示。在中国沿海水域的纤毛虫生物多样性调查中，鉴定出三种孤立的、隶属于福斯纳-施菲尔德（Foissner & Schiffmann，1975）属的多毛纲纤毛虫物种，其中包括两种新物种及一种稀有物种。浙江多毛虫（Pseudovorticella zhejiangensis sp. n.）与近缘种的主要区别在于其锥形体、明显的皮层泡、一个腹侧和一个背侧的收缩泡，以及具有三行长度几乎相等但起始位置不同的漏斗形多极性3。大连多毛虫（Pseudovorticella dalianensis sp. n.）可由其卵圆形体、一个腹侧收缩泡以及中间行最长、三行排列的漏斗形多极性3来定义。稀有物种福斯纳多毛虫（Pseudovorticella verrucosa，Dons，1915）由孙等（Sun et al.，2009）重新描述。这三种物种的小亚基（SSU）rDNA序列首次测序，对Pseudovorticella物种的系统发育进行了分析，并验证了该属的非单系性。本研究表明，形态学和基因条形码数据是揭示纤毛虫生物多样性的最佳组合。