five

Genomic Run On (GRO): determination of the nascent transcriptional rates in a series of a mex67 mutant.

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE65228
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The goal of the project was to check the effect on transcription rate of a sudden depletion of Mex67 protein by using a thermosensitive mutant: mex67-5 Cells were grown in YPD at 26ºC to 0.5 OD600. At that time cells were shifted to 37ºC to induce Mex67 protein inactivation and recovered after, 0, 5, 20, 40, 60 and 150 min.
创建时间:
2016-03-31
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