Expression data from S. cerevisiae after evolution under diverse conditions. Saccharomyces cerevisiae

We conducted a set of lab-evolution experiments in yeast and followed the long-term dynamics of aneuploidy under diverse conditions including heat shock and high PH. Overall design: Each evolution experiment starts with an ancestor strain that was subjected (in several independent repetitions) to certain growth conditions such as high temperature 39°C, permissive temperature 30°C, gradually increasing temperature from 30°C to 39°C, high pH=8.6 and normal PH. In all cases the mRNA extraction was performed on a population sample that was grown for 18 generations under stress-less conditions. This is since we aimed to measure the gene expression changes that are due to stress adaptation and not the physiological response.